Checklist Facilities and Safety

Have the staff adequately been instructed on the symptoms of infection with Mycobacterium tuberculosis, the HIV virus and other pathogens worked with in the laboratory?

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Is there a written procedure on how to act in case of a suspected laboratory associated infection/laboratory accident whereby a staff-member has potentially become infected?

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Is access to the laboratory facility controlled against unauthorized persons?

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Can the access control system, protecting the laboratory from unauthorized access, be overridden for evacuation in case of an emergency such as fire?

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Is access to first aid ensured at the laboratory?

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In laboratories where the presence of first aid providers is necessary: have enough staff members been appointed as first aid provider such that continuous presence of at least one first aid provider is ensured?

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Is the training of the first aid providers up-to-date?

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Does the Biosafety Manual contain an explanation of the tasks and responsibilities of first aid providers?

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Is the laboratory staff aware of who the first aid providers are?

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Have the Personal Job Descrptions of the first aid providers been adapted to reflect the authorizations of their position as first aid provider?

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Is there an action plan to upgrade the facilities and safety to comply with the essential TB laboratory safety requirements of the WHO TB Laboratory Biosafety Manual?

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Are primary sample collection facilities separated from the laboratory rooms?

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Is the sample processing room separated from the room where PCR is performed?

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Are laboratory rooms where incompatible procedures are being undertaken separated from each other?

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Are rooms for cleaning of laboratory glassware and tools separated from the laboratory rooms?

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Are the rooms designed and furnished in a logical way enabling a unidirectional workflow and preventing unnecessary movements of people through the rooms?

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Is office space separated from laboratory rooms?

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Is there a dedicated area for breaks/lunches?

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Is the biosafety level III laboratory equipped with a negative pressure system?

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Is the pressure of the biosafety level III regularly monitored and recorded?

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Is the negative pressure system connected to a backup electricity supply to ensure that it keeps functioning during a power failure?

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Is the negative pressure system included in the preventive maintenance program of the laboratory?

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Is access into the BSL III laboratory controlled to prevent unauthorized access?

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Has the Risk Group for each laboratory room been determined?

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Is there an action plan to upgrade the facilities and safety to get each laboratory room into compliance with the risk group defined for that room (based on the WHO TB Laboratory Biosafety Manual)?

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Is communication from the laboratory room to outside possible without leaving the room?

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Are emergency numbers available near telephones and clearly visible?

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Is the use of mobile phones forbidden inside all laboratory rooms?

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Is a primary sample collection facility available and physically separate from the laboratory activities?

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Are patients in the waiting area and primary sample collection facility protected against rain and direct sunlight?

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Does the patient sample collection facility allow for the protection of privacy of patients?

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Are there enough chairs in the waiting area and primary sample collection facility so that every patient can sit?

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Is the waiting area and primary sample collection facility accessible for physically handicapped patients?

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Does the laboratory have a procedure for processing and disposal of the different types of waste produced?

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Does the laboratory demonstrably adhere to procedures for processing and disposal of different types of waste produced?

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Are up-to-date guidelines present on safe disposal of waste?

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Does lab staff adhere to guidelines on safe disposal of waste?

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Is potentially infectious waste safely and securely stored before incineration?

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Is potentially infectious material properly incinerated?

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Are sharps securely and safely incinerated without the risk for needle accidents?

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Is the area around the fire pit/incinerator free from clutter?

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Is infectious glassware for re-use autoclaved correctly?

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Are separate, clearly distinguishable containers present for different types of chemical waste?

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Is chemical waste processed correctly?

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Are trashcans for (bio)hazardous waste clearly distinguishable to prevent confusion with the bins for normal waste?

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Is normal waste processed according to local/national regulations (if applicable)?

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Is continuous delivery of electricity guaranteed through a working generator and/or Uninterrupted Power Supply (UPS) system in case of a power failure?

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Is all the essential/critical equipment locked onto the generator?

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Are samples stored under appropriate conditions, protected from unauthorized access and demonstrably easily retrievable?

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Are all reagents and supplies stored under the proper conditions, protected from unauthorized access and easily retrievable?

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Is equipment stored under appropriate conditions protected from unauthorized use?

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Is defective equipment demonstrably contaminated and stored outside the laboratory protected from use?

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Are documents and records stored under appropriate conditions, protected from fire, insects, humidity and other harmful elements?

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Are documents and records protected from unauthorized access?

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Are documents and records stored in a logical manner so that they are demonstrably easily retrievable?

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Is an up-to-date Biosafety Manual present in the laboratory?

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Does the biological safety manual have a logical structure with table of contents, an introduction and a title page clearly stating the words “Biosafety Manual”?

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Does the Biosafety Manual contain general safety rules for working in the laboratory with human material?

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Does the Biosafety Manual contain preventive safety procedures?

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Does the Biosafety Manual contain procedures for emergency situations?

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Are the Biosafety Manual chapters signed by the author, reviewer and Laboratory Management?

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Is the Biosafety Manual easily accessible for all staff members at a logical location?

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Is the Biosafety Manual read and signed by all staff members?

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Is an indicator placed in relevant rooms for monitoring the air flow and preventing that pathogens are blown towards the staff working in the laboratory?

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Does the retention schedule for storing different types of materials include the storage durations for samples and slides?

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Does the retention schedule for storing different types of materials include the following documents:

  • Replaced SOPs
  • Completed worksheets
  • Completed log sheets
  • Completed Request Forms
  • Patient reports
  • Result registers
  • All contracts of the laboratory with external parties
  • Personnel data

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Is an SOP present describing how to clean the laboratory building?

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Does the SOP on cleaning of the laboratory building include a section with specific guidelines for cleaning laboratory rooms?

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Is a cleaning schedule present?

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Does the cleaning schedule show what should be cleaned by who and when?

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Is the laboratory cleaned on a regular basis?

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Are all hazardous materials in the laboratory registered?

Take a sample by identifying some hazardous materials and looking them up in the register.

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Is the Hazardous Material Register up to date?

Take a sample by comparing the amount of several materials as noted in the register and in reality.

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Are MSDS Sheets present for all hazardous materials in the laboratory?

Take a sample and check.

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Are there spill kits present in adequate numbers and are these kits complete

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Is there an SOP that provides procedures for dealing with the different types of spills?

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Have critical environmental/equipment parameters been identified?

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Are all critical environmental/equipment parameters monitored adequately?

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Are all Critical Parameter Log Sheets filled out completely and up-to-date?

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Are all Critical Parameter Log Sheets placed at a logical location?

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Are first aid providers present in the laboratory?

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Are first aid kits present and placed at accessible locations?

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